Laminar flow hood pdf files

In a laminar flow hood the air is passed through a hepa high efficiency particulates air filter which removes all airborne contamination to maintain sterile. The supply air is hepafiltered and flows vertically or horizontally, passing through the hood to maintain a clean work area figure 2. Aseptic technique, sterile compounding, and iv admixture. Cell culture applications utilize two types of laminarflow hoods.

Therefore biohazards or hazardous substances are not to be handled in laminar flow hoods. Quality assurancequality control guidance for laboratories performing pcr analyses on environmental samples october 2004. The captairflow laminar flow hoods provide an ultraclean and dustfree work area. How much uvlight exposure time is required to disinfect a. Laminar flow hoods provide product protection only and must not be used when working with any form of biohazard or chemical hazard any potentially infectious aerosol that is created will lead to exposure of the operator and the environment horizontalflow cleanair bench used for cell cultures can expose the researcher to aerosols of.

Biological safety cabinet management program environmental. If these substances are accidentally handled in the laminar flow hood, the risk of operator exposure is almost certain. The model nu425, class ii, type a2 laminar flow biological safety cabinet is manufactured in four sizes. Class ii microbiological safety cabinet, biohazard, made according to the en12469. Vertical laminar flow hood horizontal laminar flow hood. How much uvlight exposure time is required to disinfect a laminar flow hood. Most laminar flow hoods are available in horizontal or vertical airflow configurations. Laminar flow hoods are designed to protect the sample from out side contamination, but do not protect the operator from the sample. Also see the current version of usp, which provides some guidance on.

Diy laminar flow hood plans pg 2 this document outlines the build plans for a 2 x 4 laminar flow hood, including required materials and tools, as well as a step by step guide to assembling the cabinet. Get a printable copy pdf file of the complete article 735k, or click on a page. In vertical flow hoods, clean filtered air moves downward from the filter surface on the top to the work surface. This manual and other technical information is available in pdf format at our website. A redesigned laminar flow hood was built incorporating a vertical eyelid and a. Open flame in the cabinet will disrupt the laminar airflow in the cabinet.

Laminar flow hoods the lfhs, known as the clean benches, are designed to provide product protection only, and should not be used when manipulating infectious material. Both types of hoods use a highefficiency particulate air hepa filter and blowers that generate a nonmixing stream of air. Evaluation of laminar flow microbiological safety cabinets. Ideal for applications such as nonpathogenic cell cultures, invitro cultures. Portable vertical laminar flow hoods with adjustable blower speed feature hepa or ulpa fan that remove particulate airborne contamination to meet class 100 cleanliness requirements. Ceo, air science llc october 2016 introduction regulatory compliance procedures abound for the pharmaceutical industry, but perhaps one of the bestknown in the compounding side of the industry is usp 797. Pdf the development of a portable laminar air flow cabinet christened as steriflow was prompted by the need to perform aseptic. Labconco purifier logic series biological safety cabinet. The information contained in this manual is intended to reflect our current production standard. In horizontal laminar flow cabinets, clean air moves from a filter located behind the work surface toward operator. Usp 797 compliance in the laminar flow workstation cabinet design attributes and best practices chambre, andre.